Upright Leica SP5X White laser and spectral detection

Confocal microscope bought thanks to the FRM (medical research foundation), this system is a powerful multidimensional acquisition machine. Based on the up-to-date technology of tunable white light excitation laser, we can use any visible fluorescent dye from UV to deep red color thanks to two other lasers (405 nm diode and an classical Argon 458-477 – 488-514 nm). Equipped with Hybrid detectors that are two times more sensitive as regular PMTs, the microscope is also easily tunable in detection spectrum with the filter-free patented devices from Leica. This microscope is characterized by:

– a motorized stage in XY and Z (high speed Z-galvo stage)

– 5X, 10X and 20X dry and 40X, 63X and 100X oil immersion objectives

– 3 lasers:  405 nm diode; Argon  458-477 – 488-514 nm and a white light laser (tunable from 470 to 670 nm)

Upright Zeiss LSM 800

Confocal upright microscope Zeiss LSM 88 equipped

Lasers diodes: 405 ; 488 ; 561 and 647 nm
X-Cite fluorescent lamp (UV , green and red cubes)

Objectives:
Dry : 5X, 10X and 20X (NA 0.8)

Water Immersion ( long working distance : patch-clamp type): 20x (NA 0.5) , 40X (NA 0.8) , 63X (NA 0.9 )

Oil Immersion : 40 X (NA 1.4)

Upright Olympus Fluoview FV500

Olympus confocal upright microscope for fixed tissue :

Lasers:

Argon : 488 nm : FITC, Alexa 488, GFP , YFP …

He / Ne 543 nm : Cy3 , Alexa 543 , RFP …

He / Ne 633 nm : Cy5 , Alexa 633, deep red  RFP ( plum … )

Hg fluorescent lamp HBO 100W (UV , green and red cubes)

Objectives:

Dry : 4X , 10X (NA 0.4) and 20x (NA 0.7)

Oil immersion: 40X (NA 1.0 ) 60X (NA 1.4)