Probing the efficiency and polarity of perisomatic GABAergic transmission in the adult mouse hippocampus in vivo

Inmed meeting room, Tuesday July 24th, 11 a.m.

Lab: Neurocentre Magendie, Bordeaux

Abstract: During the last decade, numerous in vitro studies have reported high(Cl−)i levels, depolarizing and excitatory actions of GABA in a wide range of pathological conditions including seizures, autism spectrum disorders, or schizophrenia. It has therefore been recently proposed that drugs restoring low (Cl−)i levels, such as the diuretic bumetanide, may provide novel therapies for a wide range of disorders. However, the relevance of this hypothesis in vivo remains largely speculative and debated, because of the limited blood brain barrier penetration and bio-availability of bumetanide in vivo, and of the technical difficulty to probe the polarity of GABAergic transmission in vivo. Combining silicon probe recordings of the firing activity of multiple individual neurons together with the detection of unitary perisomatic eIPSPs in the hippocampal circuit in vivo, we now report alterations in the interplay between excitation and time-locked perisomatic inhibition in pathological conditions such as epilepsy and neurodevelopmental disorders.

Contact R. Khazipov if you want to discuss with the speaker.